Life Sciences

Preliminary phytochemical screening, antimicrobial and gas chromatography-mass spectrometry (gc-ms) analysis of costus pictus d don

The present study was carried out to investigate the phytochemical screening, antibacterial, antifungal activity and GC-MS analysis of Costus pictus leaf extracts. Antibacterial activity of Costus pictus leaf extracts was evaluated against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and antifungal activity against Aspergillus niger, Aspergillus fumigatus, Aspergillus parasites, Candida albicans and M. Purpureus. Three different solvents Ethanol, Petroleum ether and Aqueous were used for extraction.

Phytochemical screening, gc-ms analysis and antibacterial activity of vernonia cinerea leaves

To perform preliminary phytochemical screening, GC-MS analysis and to study the antimicrobial activity of Petroleum ether, ethanolic and aqueous extracts of Vernonia cinerea leaves. Leaves of Vernonia cinerea was shade dried, powdered and extracted with respective solvents. The extracts were then screened for phytocompounds, the petroleum ether and ethanol extracts were analysed by GC-MS to detect the compounds present in the polar and non polar solvent extracts of the leaves.

Siddha medicines and methodologies on dynamic gait index of cerebral palsy children

The Objective of the study is to determine the effect of Brahmi Nei with massage and varmam on dynamic gait index of children with spastic cerebral palsy. Among the 250 children 210 Spastic CP satisfied the inclusion criteria and were divided in to three groups (N=70 Nos). Group –I treated as an active control, Group –II received Brahmi Nei, Group - III received Brahmi Nei along with external therapies such as massage with Vasavu ennai and Varmam twice a day. Experimental period was 90 days and dynamic gait index was recorded 0th day and followed by every 30th day.

Effects of auto exhaust pollution on foliar morphological structure of road side shrub species, bougainvillea spectabilis

Auto exhaust pollution serve as the major driver of the deteriorating air quality in Rewa city. This study aims to investigate the effects of auto exhaust pollution on foliar morphological structure of road side ornamental shrub species, Bougainvillea spectabilis. Increased number of stomata and epidermal cells per unit area in leaf samples of Bougainvillea spectabilis collected from polluted sites than those from control sites is observed by light microscopic technique.

Click synthesis and anti-bactrial activiy of novel tris-chalcones

Click synthesize of several novel tris-chalcone with 2,4,6-trichloro-1,3,5-triazine core from reaction of premade tris-aldhydes or tris-acetophenones with aromatic aldehydes or acetophenones under basic conditions in MeOH in the presence of NaOH are reported. All of the synthesized products were characterized by FT-IR, 1H, 13CNMR and elemental analysis. The antibacterial activities of tris-chalcones were estimated versus Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis. Some compounds display promising activities.

Synthesis and characterization of new complexes of copper (ii) and zinc (ii) a basis of schiff bases ligands n, n’-bis (diphenylmethylene) ethane- 1, 2-diamine (l1) and n, n’-bis (diphenylmethylene) benzene -1, 2-diamine (l2)

A new series of copper (II) and zinc (II) Schiff bases complexes containing the bidentate ligands N, N'-bis (diphenylmethylene) ethane-1, 2-diamine (L1) and N, N'-bis (diphenyl methylene) benzene-1, 2-diamine (L2) was prepared by the condensation of the ethylenediamine, or the orthophénylènediamine on benzophenone in ethanol at reflux. The Schiff base ligands and their corresponding complexes have been identified by IR spectroscopy, 1H NMR, 13C NMR and by ESI MS spectrometry.

Prevalence of malaria infection among children attending federal medical centre yola, adamawa state, Niageria

This study was aimed to determine the prevalence of malaria infection among children aged 6 months-15 years and considered only Out-Patient children at EPU, Federal Medical Centre, Yola from the months of June to November 2015. Ethical Clearance and Informed Consent was obtained. Questionnaires were used to gather information on age, gender of children and parents/guardians sociodemographic characteristics. Malaria was screened using Thick and Thin blood film. The prevalence of malaria infection was 29.2%. The malaria species found was P.

Studies on the effect of naphthalene balls on biochemical and histological changes in coconut pest rhinoceros beetle (oryctes rhinoceros)

To understand the effect of Naphthalene balls on Brain and Muscle of coconut pest Rhinoceros beetle was selected. The biochemical parameters like Protein, Glucose and Glycogen on Brain and Muscle of both control and Naphthalene balls exposed Rhinoceros beetle have been carried out. The beetles were divided into two groups, one is control group (N=6). Another one is Experimental groups (N=6). The experimental group beetles were exposed with Naphthalene balls (l ball/beetle). After 96 hours of exposure the beetles in experimental group were totally dead.

Studies on gastrointestinal parasites of chicken in and around cheyyar taluk, Thiruvannamalai District

A total of 50 intestines of domestic chicken were collected in and around Cheyyar, Thiruvannamalai district from April 2014 to September 2014. The overall prevalence of parasitic infection was 91.54%. of this overall prevalence of 91.54%, 80.67% was infected with cestodes and 19.33% with nematodes. The collected tape worms (cetodes) were identified as Raillietina tetragona, R.echinobothridia, R.cesticillus, Cotugnia dionophora and Hymenolepis carioca) and nematodes were identified as Ascaridia galli and Heterakis gallinarum.

Investigation of effect one. coli of food dyes and lacz gene expression

This study aimed to investigate the effect of Brilliant Blue and Dark Green food dyes on the Escherichia coli which is part of the intestinal flora.The food dye stock solution was prepared as 50 mg/ml with serum physiologic solution. Both of the food dyes were added in nutrient broth medium as 5, 2,5 and 1,25 mg/ml concentrations Design bacteria was inoculated inside of the dyes a 0.34 McFarland. After samples werewaitedin shaking incubator during 24 h, streaking was made to agar medium. In addition, RNA of control and sample groups were extracted forusing kits for molecular studying.