PCOS is of significant incidence in the females of reproductive age group. The easier modes of diagnostic techniques have created a unusual scenario of PCOS in the present day status. Aim: To study the hormonal imbalance and Insulin resistance in patients of PCOS. Materials and Methods: This is an observational study conducted in the clinical laboratory of Dept of Biochemistry, Maharajah’s Institute of Medical Sciences , Nellimarla and Sri Venkateswara Medical College, Tirupati. 50 subjects were studied over a period of 9 months after dividing them into two groups based on their body mass index. Results: Insulin Resistance was found to be higher in those with BMI > 25, when compared to the subjects with BMI < 25. The results were statistically significant. High Insulin Resistance influences the hormonal status of the individuals.