Durian nursery can be done by using source of generative and vegetative material. Both sources of propagation have their own weaknesses and strengths. To obtain quality seeds, the combination of two sources of propagation materials each has complementary advantages. The process of merging can be through grafting or bud buds (grafting). Connection method on durian nursery can use side grafting and bud grafting. The purpose of this research is to know the difference of carbohydrate, protein, lipids, and auxin content in various sources and storage times scion. Factorial and randomized blok design was selected in the present study. The first factor was the sourcesof scion (S), consiting of three source of stems. The stemswere primary (S1), seconday (S2), and tertiary (S3) stem. The second factor was stoage times of scion (T), comprising four level of time. The levels wee 0 day (T0), two days (T1), four days (T2), and six days (T3). Overall, there were 12 treatments in the study. It was concluded that Primary Branches, Secondary Branches and Tertiary Branches have a downward trend in line with the storage times of scion of Carbohydrate Content, Lipids Content, Water Content, and Auxin Contents unless the Protein Content tends to increase in line with the storage times of scion. The highest percentage of live grafting is influenced by carbohydrate content. The highest percentage of dorman scion is influenced by water content, the highest shoot rate is influenced by water content, the highest shoot length is influenced by water content, the highest leaf number is influenced by water content, the highest stem diameter is affected by Auxin , Leaf Area is highest influenced by Auxin, Nisba Leaf Area is highest influenced by Water Content, and Nisba Pupus The highest root is influenced by Moisture Water. Carbohydrates, Proteins, Highest Water Content is found in the Secondary Branch and Auxin is highest in Primary Branch.