Objectives:-To identify the prevalence of catheter associated urinary tract infection in critical care unit. To formulate structured urinary catheterization checklist and implement in the hospital. To evaluate the effectiveness of urinary catheterization checklist to reduce catheter associated urinary tract infection. Methods: - An experimental research was conducted on 300 samples in Dr. L H Hiranandani hospital. Non probability purposive sampling technique was used for the study. Urinary catheterization checklist implemented. Checklist was used to collect the data. Result: - 80% of all intensive care unit patients are treated with urinary catheter. Catheter associated urinary tract infection caused by improper handling of catheter, not using aseptic techniques while insertion and collection of urine. In this study a urinary catheter checklist is implemented to check the insertion technique, to maintain the catheter properly and to reduce the incidence of CAUTI. Rate of catheter associated urinary tract infection before implementation of urinary catheterization checklist was higher that is 5.33 % and it is decreased to 1.33% after implementation of checklist. Conclusion: - Urinary tract infection is a major lead to hospital associated morbidity and mortality. 32%of hospital associated infections and 13% of death due to catheter associated infections are noted. There are many risk factors which is causing urinary tract infection. But the major contributor for infection is presence of urinary catheter. CAUTI is mainly occurred when the patient care is compromised. Reduction of incidence rate should focus on identifying the high risk patients. Urinary catheterization checklist focused on identification of high risk patients and therefore reduction in the CAUTI incidence.