To understand the effect of Naphthalene balls on Brain and Muscle of coconut pest Rhinoceros beetle was selected. The biochemical parameters like Protein, Glucose and Glycogen on Brain and Muscle of both control and Naphthalene balls exposed Rhinoceros beetle have been carried out. The beetles were divided into two groups, one is control group (N=6). Another one is Experimental groups (N=6). The experimental group beetles were exposed with Naphthalene balls (l ball/beetle). After 96 hours of exposure the beetles in experimental group were totally dead. The beetle in the control group untreated with any chemical, were showed healthy. After 96 hours of exposure the beetles from both control and experimental group were dissected out for the analysis of biochemical and histological studies. The concentration of protein in Brain and Muscle of control group of beetle showed 11.52±0.3µg/mg in brain, 26.26±0.1µg/mg in muscle. Bat the protein concentration in brain and muscle of experimental group of beetle treated with Naphthalene balls showed decreased level 9.23±0.3 (p<0.05) µg/mg in brain and 12.52±0.1 (p<0.05) µg/mg in the muscle. The concentration of Glucose in brain and muscle of control group of beetle showed 1.354±0.8 µg/mg in the brain and 0.924±0.1 µg/mg in the muscle. Where as in the experimental group the Glucose concentration was reduced 0.812±0.7 (p<0.05) µg/mg in the brain and 0.312±0.1 (p<0.05) µg/mg in muscle, were noticed. (p<0.05) statistically Significant. The concentration of glycogen in the brain of control group showed much higher level 2.866±0.9 µg/mg and muscle showed 1.473±0.1 µg/mg. but in the case of Experimental group the glucose cotent was lesser when compared to control group 1.256 ±0.8 µg/mg in Brain and 0.711±0.1 µg/mg in muscle. The brain of control group of beetle showed normal histology outer nerve fibers inner granular layers and neural bundles were arranged uniformly, in the case of experimental group of beetle treated with Naphthalene balls showed broken of neural bundles, scaterly arranged glial cells and disturbance of glandular layers were seen. On the other hand the muscle of control group beetle showed uniformly arranged dark and light bands with connective tissue. The sarcoplasm and sarcolemma are clearly arranged, but in the case of experimental group of beetle the muscle showed broken of dark and light bands, unevenly arranged sarcolemma of sarcoplasm. This may be due to exposure of naphthalene balls.
