The present study was carried out to investigate the phytochemical screening, antibacterial, antifungal activity and GC-MS analysis of Costus pictus leaf extracts. Antibacterial activity of Costus pictus leaf extracts was evaluated against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and antifungal activity against Aspergillus niger, Aspergillus fumigatus, Aspergillus parasites, Candida albicans and M. Purpureus. Three different solvents Ethanol, Petroleum ether and Aqueous were used for extraction. Phytochemical analysis revealed the presence of active ingredients such as Alkaloids, Steroids, Terpenoids, Glycosides, Tannins, Saponins, Phenols, Flavonoids and Proteins. GC-MS analysis of Petroleum ether and Ethanol extract indicated the presence of phytoconstituents such as Quercetin 7, 3, 4’-trimethoxy, Squalene, Hexadecanoic acid, Octadecanoic acid and Phytol. All the extracts exhibited different degrees of antimicrobial activity against selected microbes, among the extracts Ethanolic extract showed maximum zone of inhibition 18.017±0.076mm against Salmonella typhi and aqueous extract exhibited highest fungal activity 15.033±0.058mm against M. Purpureus. It is evident from the study that C.pictus has highest therapeutic potential due to the presence of rich amount of phytoconstituents along with antimicrobial activity.
