India boasts a number of species of edible leafy vegetables, some of which have no English names and are only recognised by their native names. India is blessed with a range of natural environments, diverse climates and varying seasons. These leafy veggies are consumable and are also known as unconventional greens or underutilised leafy vegetables. These neglected greens are affordable, simple to prepare and packed with vitamins, minerals, protein, and other nutrients. The presence of poisonous and anti-nutritional principles is the fundamental issue with nutritional exploitation of underused greens. Phytates, nitrate, oxalate, and saponin are frequently found in high concentrations in leafy greens. The present study was undertaken to separate, identify and quantify each nutritional components by using standardized analytical methods, which also includes usage of HPLC-LCMS technique, used to separate, identify and quantify each component and Gas Chromatography-Mass Spectrometry (GC-MS) technique. The investigation was carried out for biochemical profiling of underutilized leafy vegetables viz., Centella asiatica L., Alternanther asessilis L., Amaranthus cruentus L., Celosia argentea L., Commelina communis L., Oxalis carniculata L., Hibiscus cannabinus L. Water soluble vitamin profiling in selected underutilized leafy vegetables revealed highest contents of Pantothenic acid, Thiamine, Niacin, Pyridoxin, Riboflavin, Folic acid, Bioti, Cyanocobalamine respectively. Analogously, individual fat-soluble vitamin profiling in underutilized leafy vegetables revealed highest contents of Vitamin K2, Vitamin D1, Vitamin D2, Tocopherol, Vitamin K1 respectively. In view of the results obtained, it can be concluded that the selected underutilized leafy vegetables showed good biochemical constituents.
