The present investigation entitled “Evaluation of Genetic Variability for Growth and Yield characters in tomato (Solanum lycopersicum L.).” involving forty three genotypes including one check varieties of tomato was carried out in Randomized Block Design (RBD) with three replications during 2022-2023 at Faculty of Agriculture Farm, Guru Kashi University, Talwandi Sabo. The observations were recorded for growth, quality and yield characters. The maximum plant height was recorded in the genotype F-2 IDH-1 (207.67cm), days to first flowering (32.80). Maximum value of primary branches was recorded in S-25-1 (17.33), days to last fruit harvest (168.00) and number of fruits per cluster (7.67). Maximum value of days to first fruit set was observed in S-38-1 (57.33) and number of fruits per plant (90.67). Maximum value of days to first fruit harvest was observed in S-31-1 (121.67), and total soluble solids (7.23°Brix). Maximum value for pH was recorded in Sel Marmade-1 (5.17) and equatorial diameter (6.33cm). Maximum value of polar diameter (6.43cm) was observed in S-22-1. Maximum value of pericarp thickness was observed in Punjab Upma (8.00 mm). Maximum value of number of locules was found in S9-2 Cherry-1 (9.67). Maximum value of average fruit weight was recorded in S-17-1 (65.45g) and total yield per plant was recorded in S-17-1 (5.69kg). The genotypic and phenotypic correlation coefficients showed that total yield per plant had positive and significant association with number of fruits per plant, number of fruits per cluster, plant height, days to first fruit harvest, number of primary branches, equatorial diameter and number of locules. The path analysis estimates indicated that plant height has highest positive direct effect on total yield per plant followed by number of primary branches, number of fruits per plant, TSS, average fruit weight, equatorial diameter, number of fruits per cluster, number of locules, pericarp thickness, polar diameter, pH, days to first flowering, days to last fruit harvest, days to first fruit harvest and days to first fruit set.