Identifying the resistant genotypes is one of vital strategies to control Fusarium stalk rot disease in maize. Twenty-four maize inbred lines were evaluated for resistance to stalk rot caused by Fusarium verticillioides at Crop Research and Development Institute (CRDI), Vietnam Nation University of Agriculture (VNUA) during the spring of 2016, and their QTL resistance were also studied at the molecular level using SSR maker with two specific primers reference from Jun-Qiang Ding et al, 2008 reported to avoid environmental effects in the JICA laboratory, VNUA, Vietnam. Disease severity was calculated in the field using a severity scale (SS) and direct estimation of stalk discoloration (SD) by artificial inoculation method. Both SS and SD results combined molecular marker was identified 18 inbred maize lines were resistant to Fusarium stalk rot. Disease assessments based on SS, SD were significantly correlated (r = 0.85; P < 0.01) in spring, respectively. SSR marker and SD were significantly correlated (r = 0.85; P < 0.01). Eighteen genotypes showed highly resistant response in growing seasons are D1, D2, D3, D4, D5, D6, D7, D8, D9, D10, D11, D12, D14, D15, D17,D19, D20 and D21, these lines can be utilized to develop the maize hybrids resistant to Fusarium stalk rot in Vietnam.
