The present investigation was carried out in Randomized Block Design (RBD) with three replications during 2022-2023 at vegetable farm of Guru Kashi University, Talwandi Sabo. The different nitrogen level ( control, N2: 65Kg/acre, N2:75 kg/acre, N3: 85kg/acre) as basal dose was used for studying the growth and yield parameters of spinach. The result revealed that N3 (75 Kg/acre) is showing maximum plant height (32.38 cm) followed by N2 (65kg/acre). Whereas the N1: control is showing minimum 20.12 cm plant height. Maximum stem girth is recorded in N3 (1.85) followed by N2 (1.78) and N4 (1.73). Whereas the N1: Control is showing minimum stem girth. The number of leaves are maximum in N3 (12.33) followed by N2 (11.33) and N4 (10.33). Whereas the N1: control is showing 8.33 numbers of leaves. The leaf area is maximum in N3 (546.63) followed by N2 (527.40) and N4 (422.10). The fresh plant weight (g) is maximum in N3 (15.98) followed by N2 (14.62) and N4 (13.39). The maximum yield per plot is recorded in N3 (1.61 kg) followed by N2 (1.47) and N4 (1.38). Whereas the N1: control is showing minimum (0.82kg) yield per plot. Therefore the use of nitrogen-75kg/acre can able to increase the plant height, number of leaves, stem girth, leaf area, plant weight (fresh and dry) and yield.