Antimicrobial disinfectants are widely used in different food processing industry across the world. In attention to extent uses of disinfectants, it is necessary to evaluate and validate the efficacy of disinfectants and employing the minimum effective dosages. In this research the antibacterial influences of a common disinfectant solution used in food industry, quaternary ammonium compound were evaluated on six important food borne pathogens including two Gram positive (Staphylococcusaureus and Bacillus subtilis), two Gram negative bacteria (Escherichiacoli and Pseudomonas aeruginosa) and two fungus (Candida albican to Candida albicans). According to the obtained results, Bacillus subtilis was most resilient whereas the other entire test organisms were susceptible. In attention to obtained results, the used disinfectant has good antibacterial and anti fungal effects.