Foot-related ailments, such as microbial infections and discomfort due to prolonged use of footwear, have necessitated the development of functional insoles with therapeutic properties. This study focuses on the development of a medicated bamboo insole infused with Ipomoea staphylina extract, known for its potent antimicrobial and anti-inflammatory properties. Bamboo fiber was selected as the base material due to its breathability, moisture-wicking ability, and eco-friendliness. The insole was evaluated for antimicrobial activity, comfort, durability, and moisture absorption properties. Microbial inhibition tests confirmed significant antibacterial and antifungal activity, particularly against Staphylococcus aureus and Candida albicans. Additionally, wear trials indicated improved user comfort, reduced odor, and enhanced foot hygiene. The results suggest that this herbal-infused bamboo insole can serve as an innovative, sustainable, and effective solution for foot health, particularly for individuals prone to infections and excessive sweating.